Sandwich culture using bio-functional hydrogels as a three-dimensional culture model for mesenchymal stem cells

摘要

The ephrinB2-Fc was immobilized onto the protein A-conjugated hydrogels in an orientation-regulated manner. The bioactivity of ephrinB2 in the osteoblastic differentiation of MSC could be modulated by the immobilization manner. In the sandwich culture condition, MSC showed enhanced osteoblastic differentiation by activating cell signaling pathways, as seen in the 2-D culture condition.