Superhydrophobic patterned chips as platforms for high-content drug screening in 3D tissues produced in vitro

摘要

In order to establish the proof-of-concept of using superhydrophobic chips as improved and robust platforms for the hanging-drop methodology for spheroids production and drug screening, we used SaOs-2 and L929 cell lines. We tested the effect of a cytostatic drug used in clinical practice, also dispensed in a combinatorial logic in each spot of the chip. By on-chip microscopy analysis we proved the suitability of such platforms for drug screening using tumor-like models.