Morphological and textural analysis of centroblasts in low-thickness sliced tissue biopsies of follicular lymphoma

摘要

This paper presents a new method for discriminating centroblast (CB) from non-centroblast cells in microscopic images acquired from tissue biopsies of follicular lymphoma. In the proposed method tissue sections are sliced at a low thickness level, around 1–1.5um, which provides a more detailed depiction of the nuclei and other textural information of cells usually not distinguishable in thicker specimens, such as 4–5um, that have been used in the past by other researchers. To identify CBs, a morphological and textural analysis is applied in order to extract various features related to their nuclei, nucleoli and cytoplasm. The generated feature vector is then used as input in a two-class SVM classifier with £-Support Vector Regression and radial basis kernel function. Experimental results with an annotated dataset consisting of 300 images of centroblasts and non-centroblasts, derived from high-power field images of follicular lymphoma stained with Hematoxylin and Eosin, have shown the great potential of the proposed method with an average detection rate of 97.44%.