Effect of Flow Stimulation on Cultured Osteoblast

摘要

An effect of flow stimulation on cultured osteoblast has been studied in vitro. To apply the continuous shear flow on cultured cells, a cell culture dish with a donut shaped canal was contained on a shaker. Variation was made on the flow rate with the rotational speed of the shaking plate. MC3T3-E1 (osteoblastic cell line) was incubated for a week under the flow stimulation. To observe the migration of cells, the cells in the targeted area were exfoliated with a tip of a micropipette when the cells proliferated to sub confluent density. Proliferation and migration of the cells were observed with a phase contrast microscope during the cell culture. The experiments show the following results: the osteoblasts proliferate even under the continuous shear flow, and the osteoblasts migrate even to the counter direction along the flow, although osteoblasts do not show special orientation under the flow condition.