Micro Hole for Trapping Flowing Cell

摘要

Micro holes have been designed to trap a biological cell, which flows through a micro channel in vitro. Several micro traps of a half cylindrical hole of 0.002 mm depth (0.02 mm diameter) have been fabricated on the surface of the polydimethylsiloxane (PDMS) disk with the photolithography technique. A rectangular flow channel (0.1 mm depth × 1 mm width × 20 mm length) has been constructed with a silicone film of 0.1 mm thick, which has been sandwiched by two transparent PDMS disks. Two types of biological cells were used in the test alternatively: C2C12 (mouse myoblast cell line originated with cross-striated muscle of C3H mouse), or MC3T3-E1 (mouse osteogenic cell line). A constant flow (2.8 ×10~(-10) m~3/s or 8.3 ×10 m~3/s) of a suspension of cells was introduced with a syringe pump. The behavior of cells moving over the micro holes was observed with an inverted phase contrast microscope. The results show that the cell is trapped with the micro hole under the wall shear rate less than 100 s~(-1) for several seconds.