Chapter 29 Different Expression of P_(53) and Rb Gene in the Experimental Neuronal Aging with the Interference of Cholecystokinin

摘要

Objective: By using experimental neuronal aging study model, established by NBA_2 cellular serum-free culture method, we may observe the different expression of P_(53) and Rb gene in the experimental neuronal aging with the interference of cholecystokinin. Methods: Cells were assigned to AID and CCK group randomly, replaced with the above medium every other day, and then collected on day 0, day 5, day 10, day 15. Extraction the total mRNA of the collected cells, using the Reverse Transcription and Polymerase Chain Reaction technology, detect the different value of P_(53) and Rb. Results: According to the . experimental neuronal aging processes, the expressions of P_(53) and Rb gene up-regulated, and the 10D group and 15D group were lower than the 0D group; With the influence of CCK_8, the expressions of P_(53) and Rb gene had little change in every group, and there were not statistically significant among those groups. Conclusion: According to the experimental neuronal aging processes, the expressions of P_(53) and Rb gene were up-regulated. It was speculated that P_(53) and Rb gene might be involved in the aging processes; With the influence of CCK_8, the aging has been delayed, and it could depress the expressions of P_(53) and Rb gene. A tenable hypothesis is that the genes are involved in the development of aging, and CCK can delay the aging processes through down-regulating the P_(53) and Rb gene.