Simultaneous Determination of Atractylenolide Ⅱ and Ⅲ in Rhizoma Atractylodes Macrocephalae and Chinese Medicinal Preparation by Reverse-Phase High-Performance Liquid Chromatography

摘要

This present paper describes the development of a reverse-phase high-performance liquid chromatography (RP-HPLC) coupled with UV spec-trometry for simultaneous determination of atractylenolide Ⅱ and Ⅲ in traditional Chinese medicines and Chinese medicinal preparation. The standard of atractylenolide Ⅱ and Ⅲ was isolated from Rhizoma Atractylodes Macrocephalae. From a variety of solvents tested, methanol was found to be the best solvent for simultaneously extracting atractylenolide Ⅱ and Ⅲ from Rhizoma Atractylodes Macrocephalae and Chinese medicinal preparation samples. RP-HPLC analysis of the extracts was performed on an analytical column (SHIMADZU ODS, 150 × 4.6 mm; i.d., 5 μm) equipped with a security guard pre-column system. The results showed that there was a good linearity over the range 0.5-50.0 μg/ml (r ＞ 0.99). The recoveries were more than 95.0 % in Rhizoma Atractylodes Macrocephalae, and the intra- and inter-day coefficients of variation were less than 5.0 % in all cases. The lower limit of quantification (LLOQ) was 0.5 μg/ml. The RP-HPLC method was readily applied to simultaneously quantitate atractylenolide Ⅱ and Ⅲ in Rhizoma Atractylodes Macrocephalae and Chinese medicinal preparation (Shenlingbaizhu granule).