Multi-functional Polymeric Micelle Delivery System for Drug Resistant Cancer Treatment

摘要

We demonstrated that PgP polymeric micelle is a promising carrier for both plasmid DNA and siRNA capable of transfecting breast cancer (MCF-7) cells in 10% serum condition. We also demonstrated that transfection duration is long lasting. Currently, we are preparing folate (FA) conjugated PgP (FA-PgP) to evaluate the receptor-mediated intracellular delivery of FA-PgP/pGFP complex using MCF-7 cells over-expressing folate receptor and MBA-MB 468 deficient in folate receptors. In the future, we will use siRNA targeting P-gP to knockdown P-gP expression in doxorubicin resistant MCF-7 cells.