A sequential double non-flourescent immunostaining for the easy identification of the motor component in the in vivo study of biomaterials for peripheral nerve regeneration

摘要

The routine combination of myelin basic protein (MBP) and choline acetyltransferase (ChAT) immunostaining provides a highly specific, highly contrasted paraffin-embedded sections where the optical differentiation of myelinated motor fibers is easy and straightforward. This new staining protocol retains the advantages of paraffin embedded sections such as (ⅰ) having a relatively simple methodology, (ⅱ) years-long storage life, and (ⅲ) easy sharing among laboratories. This method will likely speed-up the routine histological study of nerve regeneration and will contribute a better identification of the nerve motor component.