Mineralized E1001(1k) Scaffolds for Bone Tissue Regeneration: The Effect of Dicalcium Phosphate Dihydrate Composition on Human Dental Pulp Stem Cells

摘要

The process to fabricate E1001(lk)/DCPD scaffolds using a combination of techniques was optimized. In vitro study (up to 7 days) showed that performance of DCPD scaffolds is as good as that of P-TCP scaffolds based on the results of F-acting staining. Additional characterizations are being performed to investigate the cell differentiation as well as to scale up the scaffolds to medium and large animal models. Considering previously reported results, we hypothesized that by 28 days of cell culture, DCPD scaffolds can perform better with a higher differentiation rate. This is based on the fabrication procedure adopted for the scaffolds and that because DCPD is one of the precursors of biological apatite, it can help in enhancing the osteoconductivity of scaffolds.