Development of a bi-modal, in situ crosslinking method to achieve multi-factor release from electrospun gelatin

摘要

This work describes a method for multi-factor delivery with distinct release kinetics from electrospun gelatin through the development of two in situ crosslinking techniques. A photocrosslinking method enabled encapsulation of factors that were released as a function of diffusion. A second reactive crosslinking method facilitated crosslinking of protein within the gelatin fibers that were released as the fibers degraded. Cumulatively, both systems displayed tunable release rates over a wide range. In situ crosslinking enabled the preprogramming of release kinetics that were used to demonstrate controlled multi-factor delivery from a single mesh through co-electrospinning. Future work involves confirming bioactivity retention of electrospun growth factors.