Utilizing Injectable Decellularized Extracellular Matrix Hydrogels for the Slow Release of MicroRNAs

摘要

Conclusions: ECM hydrogels resulted in a steady release of the miRNAs over a two-week period in vitro. Slower release of the antagomir was likely due to electrostatic interactions between the attached cholesterol group and ECM hydrogel, as indicated by further release of the antagomir following incubation with 1.5M NaCl. In addition, the released antimir and antagomir did not appear to be degraded. This study establishes the ability of decellularized hydrogels to modulate the release of miRNAs, which could allow for increased efficacy by localizing the therapeutic benefits in the region of interest.