Polyethylene glycol-based Protein Nanocapsules for Functional Delivery of a Differentiation Transcription Factor

摘要

In this study, we demonstrated the design, synthesis and optimization of PEG-based degradable protein NCs. The degradable NCs can subsequently deliver MyoD to the cytosol of myoblast cells, release functional MyoD to enter the nuclei and initiate myogenic differentiation. Importantly, PEG NCs are validated as a platform which retains encapsulated protein structure and activity as evidenced by the ability of MyoD to perform complex downstream processes and regulate myogenesis. The ease of preparation, biocompatibility and effective cargo delivery make the polymeric NC a useful tool to deliver a variety of recombinant TFs for therapeutic uses.