TWISTER SEGMENT MORPHOLOGICAL FILTERING. A NEW METHOD FOR LIVE ZEBRAFISH EMBRYOS CONFOCAL IMAGES PROCESSING

摘要

We propose an extension of the classical morphological filtering based on openings by line segment structuring elements. It consists in filtering a 3D+time image with the opening by all the possible rotations of a segment in the 4D space, given an initial segment and a rotation angle. The method has been applied to remove noise from confocal laser scanning microscopy images of live zebrafish embryos engineered to fluorescently label all their cell membranes.