首页> 外文会议>International Conference on Solid-State Sensors, Actuators and Microsystems >ISOTHERMAL REAL-TIME POLYMERASE CHAIN REACTION DETECTION OF HERPES SIMPLEX VIRUS TYPE 1 ON A LIGHT-ACTUATED DIGITAL MICROFLUDICS PLATFORM
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ISOTHERMAL REAL-TIME POLYMERASE CHAIN REACTION DETECTION OF HERPES SIMPLEX VIRUS TYPE 1 ON A LIGHT-ACTUATED DIGITAL MICROFLUDICS PLATFORM

机译:等温实时聚合酶链反应检测单纯疱疹病毒型1型在光致动的数字微氟化物平台上

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We report on a light-actuated digital microfluidics platform for real-time, isothermal helicase-dependent polymerase chain reaction (PCR). This platform is used to detect Herpes Simplex Virus Type 1 (HSV-1). Reagents in droplets are individually addressed, mixed, transported and arrayed on-chip before the PCR process. 16 droplets were formed from mixing 400 nl PCR master mix droplets with 35 nl HSV-1 viral lysate droplets, arrayed into a 4×4 matrix, and amplified simultaneously, all on chip. Amplification and detection of HSV-1 can be achieved within 45 minutes. No cross contamination was observed.
机译:我们报告了用于实时,等温螺旋酶依赖性聚合酶链反应(PCR)的光致动数字微流体平台。该平台用于检测疱疹病毒类型1(HSV-1)。在PCR工艺之前,液滴中的试剂是单独寻址的,混合,在芯片上传送和排列的。 16液滴由混合400nL PCR主混合液滴形成具有35nl HSV-1病毒裂解物液滴的400nl PCR主体混合液滴,排列成4×4基质,同时放大,全部放大。可以在45分钟内实现HSV-1的扩增和检测。没有观察到交叉污染。

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