Grow of stem cells on chemical-laser generated titanium cobalt shape memory alloy nanoparticles

摘要

In this research nanoparticles are fabricated using a method that is a combination of chemical and femtosecond laser ablation methods. The resulting nanoparticles are used to coat glass substrates in order to test the biocompatibility of nanoparticles with human adipose-tissue derived mesenchymal stem cells. Nanoactuators made from nanoparticulate Co-Ti shape memory alloy show potential in mechanical stimulation of bone tissue formation from stem cells. We have represented the fabrication of Co, Ti and Co-Ti shape memory alloy nanoparticles and their biocompatibility to human adipose derived stem cells. The stoichiometry and phase transformation property of the bulk alloy is protected during attrition by femtosecond laser ablation in liquid, giving access to colloidal nanoactuators. No unfavorable effect on cell growth and attachment is observed in proliferation assay and environmental electron scanning microscopy, making this material attractive for mechanical stimulation of stem cells.