Study of Protoporphyrin Ⅸ-loaded silica hollow spheres for photodynamic therapy

摘要

The PpⅨ-loaded silica hollow spheres were fabricated using DMDES (dimethyldiethoxysilane) which could be transformed into PDMS (polydimethylsiloxane) templates , and TEOS (tetraethoxysilane) as crosslinker. The morphology of the resulting samples was observed using transmission electron microscopy (TEM), and a hollow structure was clearly observed. The PpⅨ-containing silica structure was confirmed using fourier-transform infrared spectroscopy (FTIR). The FTIR peak around 1030 cm~(-1) was assigned to the characteristic peak of silica, indicated the shell was formed by silica. The singlet oxygen generation ability of the sample was confirmed using UV-Visible (UV-VIS) spectroscopy and singlet oxygen probe (DPA, diphenyl anthracene). After combined with singlet oxygen, the photo absorption of DPA at 376 run would decrease. The resulting UV-VIS spectrum suggested that PpⅨ loaded in silica hollow spheres still generated singlet oxygen after UV irradiation.