Preliminary Investigation on an Optical Nanobiosensor Based on Au Cap-shaped Polystyrene Nanospheres

摘要

An optical biosensor with surface bound gold cap-shaped nano particles like polystyrene nanospheres has been constructed for detection of bio-molecules like antigen, antibody protein and DNA plasmid, which exhibited distinct optical properties of localized surface plasmon resonance (LSPR). It caused 1.09 nm red shift in the peak wavelength (λ{sub}(max)) of LSPR spectrum for the nanosensor exposed to c-Myc (3C7) mouse monoclonal IgG and 3.53 nm red shift to the corresponding antibody by the immunoreaction subsequently. The kinetic process for the activity of the c-Myc (3C7) antigen lost with the time has been examined. The LSPR nanobiosensor was also applied to recognition of a DNA plasmid of pCMV-Myc Mammalian Expression Vector, which would provide valuable information on molecular biology in clinical medicine, disease prediction, gene mutation and gene diagnosis.