Thermoresponsive affinity interaction between cells and immobilized antibodies on poly(N-isopropylacrylamide)-grafted surfaces

摘要

Anti-human CD90 antibody-immobilized thermoresponsive cell culture surfaces were prepared as a model for developing next-generation thermoresponsive cell culture dishes. Thymic carcinoma cells (Ty-82) didn't adhere on isotype antibody-immobilized surfaces, and the cells aggregated. By contrast, Ty-82 cells selectively adhere on anti-human CD90 antibody-immobilized thermoresponsive surfaces. This result indicated that Ty-82 adhered onto the CD90 antibody-immobilized surfaces through affinity interaction, not through nonspecific interactions. The cells were detached from the surfaces by lowering temperature to 20 °C with pipetting. Neonatal normal human dermal fibroblasts (NHDFs) were also adhered on the surfaces, and formed the monolayer. By reducing the temperature to 20 °C, NHDFs were spontaneously detached as a single cell sheet. In conclusion, antibody-immobilized thermoresponsive cell culture surfaces for regulating selective cell adhesion were successfully prepared. This surface technology would be useful for selective cell attachment/detachment system such as cell separation.