Dielectrophoretic Movement of Cell around Surface Electrodes in Flow Channel

摘要

A micro How channel with the surface electrodes has been designed to detect the dielectrophoretic movement of a biological cell. The surface electrodes of titanium (thickness of 200 nm) were made along the edges of the flow channel by the photolithography technique. At the one of the edge, the tip angle of the triangle shape of the surface electrode is 0.26 rad. The other flat edge is the reference electrode. The rectangular cyclic electric stimulation (0.01 ms, or 0.001 ms of period) was applied between the surface electrodes. The suspension of C2C12 (mouse myoblast cell line originated with cross-striated muscle of C3H mouse) was introduced to the flow channel, and the flow rate was controlled by the pressure head between the inlet and the outlet. The experiment shows that the velocity of the cell is accelerated along the electric field during the flow through the flow channel.