Electrospun fibers with loadings of calcium phosphate/plasmid DNA nanoparticles as potential scaffolds for vascular tissuse engineering

摘要

Reverse microemulsion was used in this study to synthesize CaP/pDNA nanoparticles, which showed a high transfection efficiency on HUVECs. Biodegradable electrospun fibers loading of CaP/pDNA nanoparticles maintained a sustained release of pDNA. In vitro transfection efficiency study indciated the efficent expression of VEGF and bFGF. In vivo subcutaneous implantation of pDNA-loaded fibrous scaffolds promoted blood vessel formation, indicating potential scaffolds for vascular tissue engineering. This work was supported by National Natural Science Foundation of China (51073130) and National Basic Research Program of China (973 Program, 2012CB933602).