Efficient GLP-1 gene delivery using two-step transcription amplification system with a signal peptide and arginine-grafted bioreducible polymer

摘要

TSTA system showed higher GLP-1 gene transcription than mono pDNA. Attached SP can improve the expressed GLP-1 secretion level after amplification of transcription in TSTA-SP system. ABP displayed higher GLP-1 gene delivery efficiency than PEI25k. Finally, GLP-1 from ABP/TSTA(SP-GLP-1) polyplex medium-treated cells can induce the highest insulin secretion in insulinoma cells, meaning this novel system could be utilized as a safe and efficient GLP-1 gene delivery system.