Degradation and bioactivity studies of drug loaded porous scaffolds for bone regeneration applications

摘要

The scaffolds of polymethylmethacrylate (PMMA) and poly (1,4-butyl succinate) extended 1,6-diisocyanatohexane (PBSu-DCH) polymer with silicon dioxide and hydroxylapatite (HAp) nano particles have been prepared in the laboratory for the bone repair applications. Nano particles of silicon dioxide and HAp have been prepared by using Stober and co-precipitation methods and cubic scaffold (1cm × 1cm × 1cm) has been prepared by using solvent casting method. PMMA is non-degradable polymer. In order to improve the degradation of scaffolds, PMMA and PBSu-DCH have been added (0,10,20 w/v %) in pure chloroform with 2 wt % of silicon dioxide and 2 wt % of HAp nano particles. Porous scaffolds have been investigated with the help of X-Ray Diffraction, Fourier Transform Infrared Spectroscopy and Field Emission Scanning Electron Microscopy studies. Biodegradability of scaffolds has been checked with two most encountered buffer solutions found in human body i.e. phosphate buffer saline (pH 7.4) and citric acid buffer solution (pH 3). It has been observed that biodegradability of scaffolds has increased with the addition of synthetic polymer (PBSu-DCH). Prepared scaffolds have been observed to bioactive in nature with the formation of new HAp phase within 3 days of in vitro analysis. Moreover, scaffold, has been observed to be nontoxic which provides healthy environment for the growth of human osteoblast MG 63 cells. Prepared scaffolds have advantages of excellent bioactivity and tendency to kill the microorganisms.