Integration of Multi-Focal Multi-Photon Microscope and Second Harmonic Generation for 3D High-Resolution Imaging of Liver Fibrosis

摘要

In conclusions, we have developed an imaging system which is an integration of MMM, SHG and an automated slicing module for large tissue imaging. The automation and 64-times imaging speed with label-free SHG offers an attractive tool for cross-scale high throughput imaging which provides opportunity to study spatially changes of ECM and tissue structures in liver fibrosis.