Preparation of pectin-based nanosized drug delivery system with diverse morphologies and their targeting antitumor effect on hepatocellular carcinoma cells

摘要

This study was aim to find out the best morphology that have the best targeting on hepatocellular carcinoma cells. The drug-loaded pectin nanospheres, vesicles and nanorods were fabricated in aqueous media containing Ca~(2+) and CO_3~(2-) ions. Through adjusting the preparation conditions, nanosized drug delivery system with diverse morphologies, that is, nanospheres, vesicles and nanorod could be obtained. The cell survival rate were determined by Trypan blue staining method. CCK-8 method was used to study the inhibition effect of 5-FU, drug-loaded pectin. Nanospheres, vesicles and nanorods and blank drug loaded systerm on HepG2 cell line. Cellular uptakes were determined by HPLC method. DNA fragments by agarose gel electrophoresis, flow cytometry and Hoechst staining were used to confirm the death of the HepG2 cell. The nanospheres have the best targeting and antitumor effect on hepatocellular carcinoma cells.