In vitro Cellular and Molecular Mechanism of Osteoinductivity by Silicon Substituted Hydroxyapatite

摘要

In conclusions silicon substituted hydroxyapatite was shown to enhance the bioactivity of hMSCs in vitro through its chemical composition irrespective of the influence of osteogenic supplements or surface morphology. The mechanism of osteoinduction involves the chemical composition of SiHA on expression of osteogenic marker genes and secretion of the osteogenic proteins. SiHA was shown to induce osteogenesis via early osteogenic differentiation of hMSCs as a result of the early termination of cell proliferation and early initiation of bone matrix mineralisation through increased expression of osteonectin gene and production of osteocalcin protein. Early expression of VEGF indicates the contribution of SiHA and HA to the promotion of early neo-vasculogenesis. In order to further evaluation of this biomaterial as an osteoinductive biomaterial, an in vivo study of bone formation by SiHA needs to be carried out.