Staphylococcus epidermidis expressing green fluorescent protein to study biomaterial-associated infections in mice

摘要

An S. epidermidis O-47 strain was constructed which constitutively and stably expresses GFP at a well-detectable level. The plasmid was retained without selection pressure in vitro and in vivo, and expression of GFP had no influence on the fitness in vivo. Therefore, S. epidermidis O-47 GFP is a useful tool for in vivo analysis of bacterial survival in experimental biomaterial-associated infection.