Determination of Chitosan Oligosaccharide Content by Acetylacetone Spectrophotometry Method

摘要

Objective:To establish a common method to detect the content of chitosan oligosaccharide.Methods:Chitosan oligosaccharide was hydrolyzed completely by concentrated hydrochloric acid,and the solution was regulated into neutral with NaOH.Then,determined the absorbance in 525nm,and substituted into the regression equation to determine the results.Results:The results showed there was a good linear relationship when the concentration of chitosan oligosaccharide ranged from 0.02 mg/mL to 0.12 mg/mL,r2 =0.999.The average recovery of chitosan oligosaccharide samples was 99.25％.Conclusion:The method is sensitive,accurate and simple.It is applied to determine of the content of chitosan oligosaccharide.