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Optical-digital restoration of out-of-focus color images detected in microscopy

机译:在显微镜下检测到的离焦彩色图像的光学数字恢复

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An optical-digital method is proposed for restoring trichromatic images out-of -focus, detected with a monochromatic CCD. The optical system (OS) is a microscope working in bright field mode and the source of illumination is polychromatic. To carry out the restoration, an estimated of the intensity point spread function is obtained from the image of a non-resolved object (quasi-point source) by the OS in three wavelengths. From the image of the quasi-point source, its spectrum is calculated for obtaining the optical transfer function associated with each wavelength. The Wiener filter is built with the spectrum, and the restoration is made for each color. By an addition of the three restored images, an estimated of the trichromatic object is recovered. The obtained results and conclusions are presented.
机译:提出了一种光数字方法,用于恢复用单色CCD检测到的散焦的三色图像。光学系统(OS)是在明场模式下工作的显微镜,照明源是多色的。为了进行恢复,通过OS在三个波长下从未分辨物体(准点源)的图像获得强度点扩展函数的估计。从准点光源的图像中,计算其光谱以获得与每个波长相关的光学传递函数。维纳滤光片是用光谱构建的,并且针对每种颜色进行了还原。通过添加三个恢复的图像,可以恢复三色目标的估计值。给出了获得的结果和结论。

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