Optical interference for multiplexed, label-free, and dynamic biosensing: Protein, DNA and single virus detection

摘要

Direct monitoring of primary molecular binding interactions without the need for secondary reactants would markedly simplify and expand applications of high-throughput label-free detection methods. We have developed the Interferometric Reflectance Imaging Sensor (IRIS) for label-free, high throughput, high sensitivity and dynamic detection of molecular binding on a solid surface. IRIS has demonstrated protein-protein binding and DNA-protein binding in real time, label-free, and in a high-throughput format with exquisite sensitivity (∼10 pg/mm²) and reproducibility [1,2] as well as label-free measurements of DNA hybridization kinetics [3] and viral detection [4].