PLCL/collagen composite nanofibrous scaffolds for bone yissue engineering

摘要

To mimic natural bone extra cellular matrix (ECM), electrospun nanofibers with good biocompatibility have attracted a lot of attentions in recent years. Collagen type I as a main composite of natural bone tissue has been fabricated into nanofibrous mats and employed in bone tissue engineering. In this study, Human fetal osteoblasts (hFob) were seeded on the scaffolds to study their proliferations and differentiation. The proliferations and alkaline phosphatase (ALP) activity of hFob at 3, 7 and 14 days were investigated. The morphology of the composite nanofibers and cells was characterized by scanning electron microscopy (SEM). Hydrophilic property of the scaffolds was studied through test of water contact angle. The proliferations of hFob were evaluated by MTT method and ALP activities were measured by using the Anaspec test kit. The results showed Collagen Type I enhanced the proliferations and ALP activities of hFob. PLCL/Collagen composite nanofiberous scaffolds may have a great potential for bone tissue engineering.