Protein and Polyphenols Contaminants of Sodium Alginates and Their Effect on Viability of Mesenchymal Stem Cells Derived from Human Umbilical Cord

摘要

Alginic acid is a linear polysaccharide that mainly consists of two building blocks of mannuronic (M) and guluronic (G) acid residues. This biomaterial is frequently employed in variety of biomedical and pharmaceutical applications such as dental impressions, wound dressing and controlled drug delivery1. Due to their ionogeling property (ionotropic gelation), excellent biocompatibility and pliability, alginate gels are particularly suitable as scaffolds for tissue engineering applications. Apart from biocompatibility and batch-to-batch reproducibility, a successful clinical trial for such applications requires the alginate material to be pure. Proteins and polyphenols are the most common impurities in the pharmaceutical grade alginate polymers2, and the use of such polymers as a tissue engineering scaffold requires a comprehensive evaluation of the contaminants and their role in cell viability.Objective of the study: To determine the amount and the effect of proteins and polyphenols of pharmaceutical grade sodium alginates on cell viability of Human Umbilical Cord Cells (hUMSCs).