Development of Automatic dual-channel SPR detecting instrument

摘要

A self-developed automatic dual-channel detecting instrument based on SPR (surface plasmon resonance) principle was presented in this paper. This instrument was constructed by the sample-load system, the sample flow-routing system, SPR sensor and control circuit. Formed by a sample injection needle that can move up and down and a sample plate, the sample-load system can realize the measurements of 15 kinds of samples, and the automatic cleaning of the sample injection needle. The sample-routing system consisted of two precision syringe pumps and a microelectric valve, which ensured that there is always solution flowing through the SPR sensor surface during the automatic sample injection process. The whole sampling process was jointly accomplished by sample-load system, the sample-routing system and the control circuit. Applying two section of air to isolate, it separated the sample from the buffer or the regenerated liquid, and then reduced the instrument's measurement error. Sensor is the kernel of the whole instrument, and a self-designed dual-channel SPR sensor was applied to this detecting instrument. The control circuit mainly included: the MCU(microcontroller unit) control unit with the core of C8051F020 to control the automatic sampling system of pumps and valves, USB2.0 interface circuit with the core of CY7C68013 to realize the dual-communication between PC and MCU, the real-time data acquisition and uploading to the PC for further analysis and processing. The results of the concentration detecting experiments and data analysis of sucrose showed that this system has good measurement accuracy and stability.