Antigen Delivery System by Combination of Bubble Liposomes and Ultrasound for Cancer Immunotherapy

摘要

We investigated the feasibility of the combination of bubbleliposome and ultrasound exposure for DC-based cancer immunotherapy. OVA was directly delivered into the cytosol but not via the endocytosis pathway, and OVA-derived peptides were presented on MHC class I. This result indicates that exogenous antigens can be recognized as endogenous antigens when delivered into the cytosol. Immunization with DCs treated with OVA, BLs and US exposure efficiently induced OVA-specific CTLs and resulted in the complete rejection of E.G7-OVA tumors. It was indicated the delivery system by BLs and US is useful for DC-based cancer immunotherapy.