We are developing beta 1,3-D-glucan hollow, porous 2-4 micron particles (GP) as a dectin-1 receptor-targeted delivery system for the co-delivery of antigens, adjuvants and siRNAs. GP encapsulated antigens provide for more effective targeted delivery to dendritic cells (DCs) compared to free antigen in in vitro T-cell proliferation assays, and robust in vivo induction of humoral and T-cell responses compared to the adjuvant alum. Addition of CpG as an adjuvant further enhanced cytokine release, immune responses and induced a Th2 to Thl antibody shift. We are currently developing GP formulations to co-deliver antigen, adjuvants and siRNA using siRNAs targeting immune suppressors to further increase the effectiveness of GP vaccines.
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