CULTURE OF WHITE-ROT FUNGUS CORIOLUS VERSICOLOR AND POLYMERIZATION OF 2,4-DICHLOROPHENOL CATALYZED BY THE RELEASED LACCASE

摘要

Chlorophenols and its derivatives which are typical toxic organic pollutants are discharged into environment mainly from effluent of chlorine- containing bleaching in the paper industry and biocides production process. In this work, 2,4-dichlorophenol (2,4-DCP) as typical chlorinated aromatic compounds was polymerlized with catalyzer of laccase from a white-rot fungus, i. e. Coriolus versicolor. The polymerization process of 2, 4-diphenylphenol catalyzed by the crude laccase was analyzed. The results indicated that more than 93.6% of the chlorophenols was removed after laccase treatment in a suitable condition for 20h. The molecular weight of the obtained products was analyzed with gel permeation chromatography (GPC). The result showed that the average molecular weight of polymerization is 33.8 KDa. The Chemical structure of the product was also analyzed by FT-IR and 13C-NMR. As a conclusion, the laccase from Coriolus versicolor has a high ability to polymerize 2,4-chlorophenols. It is suitable for wastewater treatment from chlorine based bleaching process.