Enhancement of Fermentative Lactic Acid Bacteria on Decolorization of Palm Oil Mill Effluent Using Nutrient Supplement

摘要

After anaerobic treatment of palm oil mdl effluent (POME), color and phenolic compounds still remain and require for further treatment to remove these pollutants that affected in micro and macro-aquatic life. Biological treatment, an environmental friendly mean, was applied in this study. Five strains of lactic acid bacteria (LAB) were used to screen the abdity in decolorization of palm oil mill effluent Among them, Lactobacillus plantarum showing the highest decolorization yield and reduction of phenolic compounds were 16 % and 34 %, respectively. This L. plantarum was isolated from Thai spoilage fruit. The nutrient supplement using glucose and yeast extract as carbon and nitrogen sources for enhancing L. plantarum in decolorization and reduction of phenolic compounds in treated POME were studied. Treated POME (TPOME) was obtained from the effluent of anaerobic pond system of palm oil industry and used in this study. The effects of glucose and yeast extract were studied in the range of 0.5-2.0% and 0.1-0.4% (w/v), respectively. The experiments were carried out in I L Duran glass laboratory bottle (Duran(@), Germany) containing sterilized TPOME and incubated at 30°C under anoxic condition for 7 days. It was found that L. plantarum showed the maximum decolorization yield (54 %), phenolic compound reduction (42 %) and COD removal (47 %) by supplement 1 % glucose and 03 % yeast extract. The results of decolorization yield and phenolic compound reduction were higher than TPOME without nutrient supplement in 43% and 25%, respectively, as well as the cell growth was more than 5 times. It was noticed that decolorization and phenolic compound reduction in TPOME were directly depended on the cell growth.