Study on Inter-relationship of the Nutrients in Substrate and the Production of Demulsifying Compound by a Bacillus sp.

摘要

This work investigated the effect of substrates on de-emulsification capability and the growth of a bacillus sp.(screened by our laboratory).The biomass and de-emulsification ratios(DR) of cultures grown in substrate with the different nutrients (glucose,liquid paraffin,NH4NO3 and yeast) were determined.In the orthogonal tests,the significant of de-emulsification capability in turn is glucose>liquid paraffm>yeast>NH4NO3.The glucose is the highest effect of nutrients on de-emulsification capability and growth.The de-emulsification ratios(DR) and the glucose utilization ratios increase simultaneously at log and lag growth phase.Measurements of the liquid paraffin residue indicates that 30.1% liquid paraffin in substrate is consumed and used in inducing the demulsifying compounds production rather than the increase of the biomass.The culture grown in substrate with 2%～5% liquid paraffin exhibits highest de-emulsification ratio(DR).The contents of yeast at 0.8mg/L～l.0mg/L is beneficial to the growth and demulsifying compound production.