Safety Evaluation of Gemini-Type Quaternary Ammonium Salt Antibacterial Reagent

摘要

To assess safety of gemini-type quaternary ammonium salt as a germicide, toxicity characteristics of benzyl dimethyl dodecyl ammonium chloride [BAC], 1-methyl-1-dodecyl piperazinium chloride [MDP], 1,4-didodecyl piperazinium dichloride [DDP] and l,l'-(decanedioyl)bis (4-methyl -4-dodecyl piperazinium chloride)s [DCP] were evaluated by the investigation of minimum inhibitory concentration[MIC], minimum hemolysis concentration[MC50], cytotoxicity[LC50] and acute oral toxicity. The results revealed that MIC, HC50and LC50of DCP are 1.6, 15.5 and 31.0 μmol-L-1, respectively. That of DDP are 8.3, 8.0 and 25.0 μmol-L-1, respectively. That of DMP are 24.1, 31.0 and 87.0 μmol-L-1, respectively. That of BAC are 28.9, 38.4 and 120.0 μmol-L-1, respectively. All of QACs used in the acute oral toxicity test belongs to actually non-toxic grade. It is suggest that gemini-type quaternary ammonium salt antibacterial reagent has higher safety than mono-type, and "HC50/MIC" or "LC50/MIC" as a parameter is more important to safety evaluation of QACs antibacterial reagent.