Development of chitosan-based siRNA delivery system andits use in inhibition of posterior capsular opacification

摘要

The PEGylated chitosan (CS)-based polymer carrier(BPM-PCPR) was synthesized and evaluated for efficientdelivery of small interfering RNAs (siRNAs). Ourpolymer carrier showed the significant enhancement ofcellular uptake and stability as compared to naturalchitosan. In this study, we used siRNA of NF-kappa B toinhibit posterior capsular opacity for intraocular delivery.The complexes of BPM-PCPR and siRNAs (BPM-PCPRsiRNAs)inhibited cell migration in in vitro and in vivo. Inanimal study, the BPM-PCPR-siRNAs suppressedposterior capsule opacification (PCO).