This work reports on the development of a piezoelectric DNA biosensor for the identification of enhanced Green Fluorescence Protein gene often used as a marker of gene delivery. The oligonucleotide sequence of 20 bases selected from the starting DNA has been immobilized on quartz crystal surface via biotin-streptavidin interaction. The detection of the eGFP gene was examined and optimized and the analytical parameters of the system were studied. The developed sensor was applied to the analysis of samples extracted and amplified by polymerase chain reaction.
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