Engineering Transferrin for Improved Drug Carrier Efficacy

摘要

The iron release rate of transferrin (Tf) was inhibited through replacement of carbonate with oxalate and site-directed mutagenesis. These modified Tf proteins showed increased cellular association with HeLa cells compared to native Tf. In addition, conjugates of modified Tf with diphtheria toxin (DT) showed increased cytotoxicity relative to unmodified Tf with DT (1).