Molecular cloning and functional identification of BnIND gene in Brassica napus

摘要

IND (INDEHISCENT) is a transcription factor which is required for DZ (dehiscence zone) cell differentiation and pod shattering in Arabidopsis thaliana. A homologous gene in Brassica napus (designated as BnIND) was cloned by rapid amplification of cDNA ends (RACE). Southern blot assay showed that there were three homologous loci of BnIND in genome of B. napus. The expression of the gene was restricted to the leaves, buds and pods as shown by RT-PCR method. The RNAi vector was constructed with partial BnIND and transformed to B. napus mediated by Agrobacterium to suppress the endogenous expression of BnIND. The transgenic lines were confirmed by PCR. Phenotype assay showed that the T1 generation of transgenic lines appeared disorder in DZ development.