Studied on artificial resynthesis of B. napus from B. olerecea var.aceaphala and B. camptris through embryo rescue

摘要

At present, it is one of valid methods to widen rapeseed genetic resources and resolve the problem of lack of breeding materials via artificially resynthesized Brassica napus. In these experiments, B. napus were artificially resynthesized through culture of embryos which derive from interspecies hybrid of yellow-seed B. oleracea var aceaphala(2n=C°C°=18) × yellow-seed B. campetris (2n=AR AR=20). These embryos were cultivated in 9 different culture mediums. Results showed some interspecies embryos were obtained from these crosses(05K002×05K128, 05K002×05K121 and 05K25×05K128), however, there were big different suitable mediums during embryos culture; meanwhile, most embryos became death in other crosses ( 05K018×05K12 and 05K26×05K128) about 20 days after pollination. six newly newly formed hybrids (2n=ARCO=19)with cytoplasmic of B.oleracea var aceaphala were obtained via culture of empryo, and all clones were true hybrids with 19 chromosomes via cytology test. The hybrid plants were similar to female parent in morphology in seedling period and they grow quickly. These hybrids could benefit from further improving B. napus because of their some good agricultural traits. There were some fertile plants among four clones, and the other plants and clones were sterile. All the seeds of true hybrids were black.