Genetic variation of glucosinolates in young leaves of winter rapeseed (Brassica napus L.)

摘要

Increased levels of glucosinolates (GSL) in greenmatter of rapeseed (Brassica napus L.) and other Brassica species are believed to confer resistance to certain pests and diseases. To study the genetic variation of leaf GSL and their genetic correlation with undesired seed GSL, 140 winter rapeseed accessions with a wide range of seed GSL concentrations were grown in the field.Leaf samples were taken in late fall, dried and analyzed by High Performance Liquid Chromatography (HPLC). Seed GSL ranged from 2.1 to 139.5 μmol/g drymatter (DM) ad leaf GSL ranged from 2.4 to 53.7 μmol/g DM with a correlation ofr = 0.58 (p ≤0.01 ). This indicates that there is sufficient genetic variation to select for higher levels of leaf GSL and that to a limited extent this can be done without increasing the undesired seed GSL. Leaf samples were also scanned with Near Infrared Reflectance Spectroscopy (NIRS). A NIRS calibration was developed with the recorded spectra that is suitable to conduct efficient leaf GSL analysis by NIRS for total GSL concetration (1-VR = 0.86) as well as for some individual GSL. For confirmation, a subset of 40 most informative accessions are currently evaluated in field and greenhouse experiments.