Production of improved self-incompatible lines of winter oilseed rape (Brassica napus L.) with convenient seed quality for hybrid breeding using of microspore culture technique

摘要

Doubled haploid (DH) regenerants were derived via improved microspore culture technique from crosses between the donor of "00" quality OP 2051 (pollinator) and initial self-incompatible (SI) line Tandem 6/85 with high Glucosinolate (GSL) and erucic acid content in seed. The average GSL content in this set of regenerants was 84.68 μmol/g of dry matter of seeds and the average erucic acid content was 22.84%. In the second cycle of quality improvement, two selected DH SI lines were crossed with donors of quality (two breeding materials and two commercialcultivars). The percentage of obtained SI plants was 38.2%, i.e. 87 out of 228 derived fertile DH regenerants. 72 plants were selected for the detection of erucic acid content. The average erucic acid content in the set of DH regenerants was 14.20%. Nine plants with erucic acid lower than 2% were analyzed for glucosinolate content by high-pressure liquid chromatography (HPLC) method. Five out of the selected nine plants had GSL content lower than the limit 18 μmol/g at 9% seed moisture. The results of analyses showed that in the case of SI DH regenerants from the second cycle of quality improvement the critical factor of seed quality was erucic acid content contrary to GSL content The DH method proved to be applicable for the seed quality improvement of DH lines together with maintaining of SI reaction. The method based on the spraying of inflorescences with 5% NaCl to enable reproduction of SI lines was verified and used in a large scale. In 2006,F1 seed from 44 crosses between improved DH SI lines and high yielded lines have been produced and will be included in station trials to evaluate agronomic performance in 2007.