Synthesis of Biodegradable Cross-linked Poly(?-amino ester) for gene delivery and Its Modificationinducing Enhanced Transfection Efficiency and Stepwise Degradation

摘要

Biodegradable cross-linked poly(??-amino ester)(CLPAE) was synthesized, and modified withaminohexanoic acid and lysine to CLPAE-Ahx andCLPAE-Lys, respectively for gene delivery system.They could self-assemble with pDNA formingnano-sized polyplexes and CLPAE-Ahx polyplexreleased plasmid DNA slowly through stepwisedegradation. The polymers showed minimalcytotoxicity and also high transfection efficiencies.