BIOREACTIVITY OF INDOOR PM10S AND THEIR RELATIONSHIPS WITH TRACE ELEMENT COMPOSITIONS

摘要

Plasmid DNA assay and ICP-MS were conducted to investigate the bioreactivity of PM10 and relationship between bioreactivity and trace elements of PM10 in Beijing air. Six PM10 samples were selected to represent the indoor and corresponding outdoor environments. Among six samples, the PM10 from the kitchen had the lowest TD50 (toxic dosage of PM10 causing 50% DNA to be damaged), being 45μg mL-1, suggesting the highest bioreactivity. The TD50s of two PM10 samples from smoker's homes were 100 and 263μg mL-1, indicating a relatively high bioreactivity. Trace elements are believed to be the main reasons for oxidative damage to plasmid DNA. The ICP-MS analysis combined with the DNA assay showed that water-soluble zinc had better relationship with TD50 values than other elements, implying that water-soluble zinc might play an important role in the damage of DNA.