Construction of NIH3T3 Cells Expressing PENK Gene by Retroviral Expression Vector

摘要

Objective Carrying the proenkephalin gene by recombinant retrovirus vector,to research if the gene can expressed stably in NIH3T3 cell line. Methods pLNCX2-pENK was transfected into PT67 packaging cell by Lipofectamine mediation and resistant clone was selected by G418.the viral particles that existed in the supernatants were collected, they were used to infect target cell, resistant clone was alao selected by G418.Finally, the proenkephalin gene expression was detected by immunoprecipitation and RT-PCR. Results The proenkephalin gene can integrate into NIH3T3 cell line and express established by radioimmunoassay and RT-PCR. Conclusion The proenkephalin gene can expressing stably, it could contribute to further research for the treatment of chronic pain.