In Vivo Evaluation of Plasmid DNA Encoding OP-1 Protein for Spine Fusion

摘要

To evaluate the feasibility of achieving bone formation bynon-viral gene delivery with a minimally invasive technique, plasmid or“naked” DNA encoding bone morphogenetic protein-7 (OP -1) was injectedwith a collagen scaffold into the paraspinal muscle above the L5transverse process of rats. At a dose of 25 μg OP-1 DNA, bone formationcould be detected histologically, but was significantly weaker thanthat observed with 30 μg of OP -1 protein.